Chapter: Microbes in Human Welfare

Plasmid pBR322 has Pst I restriction enzyme site within gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is

Match the following techniques or instruments with their usage.Column I Column IIA. Bioreactor (i) Separation of DNA fragmentsB. Electroph-oresis (ii) Production of large quantities of productsC. PCR (iii) Detection of pathogen, based on antigen-antibody reactionD. ELISA (iv) Amplification of nucleic acids

First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is

Choose the correct pair from the following.

Identify the wrong statement with regard to restriction enzymes.

A selectable marker is used to

The two antibiotic resistant genes on vector pBR322 are for

Match the following enzymes with their functions
Column I                                                           Column II
1. Restriction endonuclease                 i. Joins the DNA fragments
2. Restriction exonuclease                 ii. Extends primers on genomic DNA template
3. DNA ligase                                      iii. Cuts DNA at specific position
4. Taq polymerase                             iv. Removes nucleotides from the ends of DNA
Select the correct option from the following.

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

Which of the following is commonly used as a vector for introducing a DNA fragment in humanlymphocytes?

A gene, whose expression helps to identify transformed cells is known as

The DNA fragments separated on an agarose gel can be visualised after staining with

Which of the following is not feature of the plasmids?

Which of the following is a restriction endonuclease?

Which of the following restriction enzymes produces blunt ends?

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

The cutting of DNA at specific locations became possible with the discovery of

The DNA molecule to which the gene of interest is integrated for cloning is called

Which vector can clone only a small fragment of DNA?

Commonly used vectors for human genome sequencing are

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

The given figure is the diagrammatic representation of the E. coli vector pBR322. Whichone of the given options correctly identifies its certain component(s)?

 

A single strand of nucleic acid tagged with a radioactive molecule is called

For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of

Which one of the following is a case of wrong matching?

Given below is a sample of portion of DNA strand giving the base sequence on the opposite strands. What is so, special shown in it?5′— GAATTC — 3′3′ — CTTAAG — 5′

There is a restriction endonuclease called Eco RI. What does ‘co’ part in it stand for?

Which one of the following is used as vector for cloning genes into higher organisms?

Restriction endonucleases are enzymes which

The linking of antibiotic resistance gene with the plasmid vector became possible with

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

Restriction endonucleases

Manipulation of DNA in genetic engineering became possible due to the discovery of

A mutant strain of T₄ -bacteriophage R-II, fails to lyse the E. coli but when two strains R-II_x and R-II₄ are mixed then they lyse the E. coli. What may be the possible reason?

In bacteria, plasmid is

Plasmid is

Plasmids are suitable vectors for gene cloning becauseu

The process of replication in plasmid DNA, other than initiation, is controlled by

Which of the following is related to genetic engineering?

Recombinant DNA is obtained by cleaving the pro-DNA by

Genetic engineering is possible, because

Two bacteria found to be very useful in genetic engineering experiments are

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction) ?

Select the correct statement from the following.

In a mixture, DNA fragments are separated by

In recombinant DNA technology antibiotics are used

Match the following columns and select the correct option.
Column I                                                                 Column II
A. Bt cotton                                                   1. Gene therapy
B. Adenosine deaminase deficiency       2. Cellular defence
C. RNAi                                                         3. Detection of HIV infection
D. PCR                                                             4. Bacillus thuringiensis

In gel electrophoresis, separated DNA fragments can be visualised with the help of

Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements.
1. DNA is negatively charged molecule and so it is loaded on gel towards the anode terminal.
2. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
3. Smaller the size of DNA fragment larger is the distance it travels through it.
4. Pure DNA can be visualised directly by exposing UV-radiation.
Select the correct option from the following

The correct order of steps in Polymerase Chain Reaction (PCR) is

The process of separation and purification of expressed protein before marketing is called

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

The Taq polymerase enzyme is obtained from

Which of the following is not a component of downstream processing?

Stirred-tank bioreactors have been designed for

An analysis of chromosomal DNA using the Southern hybridisation technique does not use

In vitro clonal propagation in plants is characterised by

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

PCR and restriction fragment length polymorphism are the methods for

Which one is a true statement regarding DNA polymerase used in PCR?

Agarose extracted from sea weeds is used in

Stirred-tank bioreactors have been designed for

Polyethylene glycol method is used for

Gel electrophoresis is used for